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KMID : 0861020120270010041
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Korea Journal of Herbology
2012 Volume.27 No. 1 p.41 ~ p.49
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Change of Antioxidative Activity at Different Harvest Time and Improvement of Atopic Dermatitis Effects for Persimmon Leaf Extract
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Jung Woo-Young
Jeong Jong-Moon
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Abstract
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Objectives: The purpose of this study was to investigate the anti-oxidative and anti-atopic dermatitis effects of
persimmon leaf extract obtained from Cheongdo-gun, where more than 60% of Korean persimmon is produced.
Methods: Anti-oxidative effects of the crude persimmon leaf extract harvested monthly between May and
November were determined by in vitro assay using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide
dismutase (SOD)-like reaction. Anti-atopic dermatitis effects of the crude persimmon leaf extract were
determined by using collagenase type Ⅰ inhibition assay and by quantitative assays including serum histamine,
prostaglandin E metabolite and leukotriene B4 levels in animal model of atopic dermatitis using Balb/c mice.
Results: Persimmon leaf extract harvested in May had higher levels of total phenolic compounds (182.24 mg/g)
and flavonoids (23.05 mg/g) than the ones of different month extract. Also, persimmon leaf extract harvested
in May showed the most effective extract scavenging activities of DPPH free radical (13.39±0.21 μg/ml) and
superoxide anion radical (40.52±2.32 μg/ml), leading to use the persimmon leaf extract harvested in May for
the experiments hereafter. Persimmon leaf extract showed 326.71±4.6 μg/ml of 50% inhibitory concentration
(IC50) for collagenase type Ⅰ which is responsible for the degradation of extracellular matrix. In addition,
persimmon leaf extract application group could decrease serum levels of histamine, prostaglandin E metabolite
and leukotriene B4 compared to the negative control in animal model of atopic dermatitis. Especially,
persimmon leaf extract showed a significantly decreased serum leukotriene B4 level relative to the levels of
histamine and prostaglandin E metabolite.
Conclusions: Persimmon leaf extract showed anti-oxidative and anti-atopic dermatitis effects in vitro and in
vivo. These results suggest that persimmon leaf extract may have immunoregulatory function for alleviating
atopic dermatitis by decreasing collagenase activity and mast cell activation.
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KEYWORD
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persimmon leaf, anti-atopic dermatitis, anti-oxidative, 2.4-Dinitrochlorobenzen(DNCB), leukotriene
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